MagSi-Genomics


 
MagSi-DNA beads can be used as a solid phase support phase in DNA extraction and purification protocols by using a simple bind/wash/elute principle. They are intended for own development of protocols and available with a range of physical properties and a silica or carboxyl modified surface.
 
MagSi-DNA kits are meant for the extraction of genomic DNA from whole blood, saliva or vegetal material.
 
Finally, we offer a range of PCR Clean-upDNA Size Selection and Dye-Terminator removal options.

 

A Simple and Quick Way to Consistent NGS Results



 

Magnetic bead-based MagSi-NGSPREP Plus offers an efficient solution for both size selection and clean-up of the successive enzymatic reactions in library preparation for NGS applications. The simple and flexible protocol can be adjusted to your specific library preparation kit and NGS instrument. MagSi-NGSPREP Plus can be used manually but is also easy to automate for high-throughput processing. In conclusion: a simple and quick way to consistent sequencing results.

The MagSi-NGSPREP Plus kit is optimized for use on Biomek® Laboratory Automation Workstations and Hamilton® MicroLab STARline. MagSi-NGSPREP Plus allows either non-selective binding, or size-targeted binding of double-stranded DNA fragments ranging from 80 – 1000 bp with specific reagent volume to sample volume ratio’s. By increasing the volume of MagSi-NGSPREP Plus, the efficiency of binding smaller fragments increases. This enables the user to selectively keep or discard undesired fragment sizes.

MagSi-NGSPREP Plus’ flexible protocols are easy to automate for high-throughput screening.


 

Agarose gel showing 6 independent size selections from a DNA ladder, using ratios of 0.65x (left) and 1.0x (right)
DNA size distribution curves after left-sided size selection showing a direct comparison between MagSi-NGSPREP Plus and AMPure® XP. Four different bead-to-sample ratio’s were used.
 



 

High quality results

•High recovery of PCR products

  • •Excellent removal of enzymes, primers, oligonucleotides, polymerases and other contaminants
  • •Fragment size selection adjustable between 100 and 1000 base pairs
  • •Guarantees consistent sequencing results


 

Simplify your routine

•One product for all clean-up and size selection steps in the library preparation work-flow

  • •Simple bind-wash-elute procedure with short process time
  • •Protocol easily adjustable for clean-up or size selection using specific reagent-to-sample ratios
  • •Manual and automated use
  • •Compatible with standard protocols of common library preparation kits

Art.No. Product Volume
MDKT00010005 MagSi-DNA NGSPREP Plus 5 ml
MDKT00010075 MagSi-DNA NGSPREP Plus 75 ml
MDKT00010500 MagSi-DNA NGSPREP Plus 500 ml