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[Fragment Analyzer] dsDNA Reagent Kit (35-1500bp)
글번호 4 등록일 2019-04-02
등록자 운영자 조회수 438명
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Qualitative analysis of DNA requires a well separated, quality DNA ladder for sizing samples. The 100 bp DNA Ladder (FS-SLR910-0001) provides sizing from 35 bp – 1,500 via well separated ladder fragments and markers. The sizing capability provided by the ladder, shown in Figure 1, enables the analysis of multiple sample types, including microsatellites, single DNA fragments, and amplicons from multiplexed PCR.

Figure 1. A separation of the 100 bp DNA Ladder (FS-SLR910-0001) carried out on a Fragment Analyzer equipped with a Short Capillary Array (33-55) using the dsDNA Reagent Kit (35-1500bp) (DNF-910).
Single DNA fragments, regardless of source, are readily electrophoresed and sized using the dsDNA Reagent Kit (35-1500bp) (DNF-910). An example separation is depicted in Figure 2, the amplicon from a PCR of a viral sample.
Figure 2. A PCR amplicon from a viral sample separated using the dsDNA Reagent Kit (35-1500bp) (DNF-910). Capillary electrophoresis was performed on a Fragment Analyzer equipped with a Short Capillary Array (33-55). Left Panel: Electropherogram of the separation. Right Panel: Digital gel image (Gaussian Fit, Non-integrated Peaks Hidden).

Electrophoresis of multiple DNA fragments in a single sample has often proven challenging for other methods due to poor separation resolution. Products from a multiplex PCR – 18 in total – with amplicons sized between 100 bp and 510 bp, were separated as shown in Figure 3.
Figure 3. Multiplex PCR amplicons separated using the dsDNA Reagent Kit (35-1500bp) (DNF-910). Capillary electrophoresis was performed on a Fragment Analyzer equipped with a Short Capillary Array (33-55). Left Panel: Electropherogram of the 18 amplicon separation. Right Panel: Digital gel image of the 18 amplicon separation (Gaussian Fit, Non-Integrated Peaks Hidden).


The 18 fragments, sized as closely as 14 bp, were separated with distinct peaks formed for each amplicon. Such fine resolution contributes to the accurate (± 5%) and precise (2% CV) sizing of the kit. For those familiar with agarose electrophoresis, the exceptional separation resolution is perhaps best viewed via the digital gel image (Figure 3, Right Panel), bands are crisp and well separated despite the close sizes of many of the fragments.


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